WebHuman peripheral blood lymphocytes were stained with biotinylated HLA-A,B,C (clone W6/32, filled histogram) or biotinylated mouse IgG2a, κ isotype control (open histogram), … WebProduct Specific Information. This antibody will not cross-react with rabbit. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Purity is >95% by SDS-PAGE. Western blot should be performed under non-reducing conditions. It has also been successfully used in Mass Cytometry (CyTof).
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WebMHC class I Antibody (W6/32) is an IgG 2a κ mouse monoclonal MHC class I antibody (also designated MHC antibody, MHC class 1 antibody, HLA-A (Major Histocompatibility Complex, Class 1a) antibody, HLA-B (Major Histocompatibility Complex, Class 1b) antibody, HLA-C (Major Histocompatibility Complex, Class 1c) antibody, MHC class 1 heterodimer … WebArticle Snippet: Flow cytometry was performed using the following antibodies: HLA-A,B,C/Alexa Fluor488 (Biolegend, clone W6/32, 1:200), HLA-DR/PE-Cy7 (Biolegend, clone L243, 1:200), and CD274(PD-L1)/APC (Biolegend, clone 29E.2A3, 1:200). mouse MHC-I (H-2K d /H-2D d )/PE ...
WebClone W6/32 is a mouse monoclonal antibody specific to the human gene, HLA-A, or Major histocompatibility complex, class I, A. Indicated applications for antibody clone W6/32 include Western blot, immunohistochemistry, flow cytometry, immunofluorescence, ELISA, immunocytochemistry, and immunoprecipitation. ... BioLegend TotalSeq(TM)-A0058 anti ... Web(9) PeptiCHIP: A Microfluidic Platform for Tumor Antigen Landscape Identification ACS Nano October 26, 2024 Sara Feola et al. BioLegend 343306 San Diego, CA), PE-conjugated antihuman HLA-A, HAL-B, and HLA-C (clone W6/32, BioLegend 311406, San Diego, CA), and Human TruStain FcX block (BioLegend B247182, San Diego, CA). The …
WebClone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule 21. Additional reported applications (for the relevant formats) include: … WebSimplify your workflow with BioLegend’s complete set of research tools to study progenitor cells from all three germ layers, including neural and hematopoietic stem cells. Learn More. Immunology . Immunology. …
Webantibody clone W6/32 (30 min; 4 C; 30 lg/ml). Natural killer cell enrichment and KIR-typing. Polym-erase chain reaction (PCR) amplification of KIR genes and KIR-specific PCR-SSP typing of NK cell donor was performed as described previously [23] with primer pair modifications [24]. NK cells were obtained by immuno-
Web1 INTRODUCTION. Mesenchymal stem cells (MSCs) are adult SCs with self-renewal and multipotency, which can be isolated from a variety of tissues and are culture expandable. 1-5 MSCs have low immunogenicity and potent immunomodulatory properties, but no tumorigenicity. MSCs show pronounced therapeutic effects against a variety of diseases … shaolin dragon seriesWebJan 21, 2024 · A700. Application Notes : Clone W6/32 recognizes residues in the N terminus of the human 2-microglobulin molecule 21 . Additional reported applications (for the relevant formats) include: immunoprecipitaton 2 , Western blotting (non-reducing) 3 , immunohistochemical staining of acetone-fixed frozen tissue sections 4,5 , blocking 6,7 ... shaolin dynasty movies english subtitleWebNov 1, 2024 · The subunit composition of the PLC was verified by using the antibodies anti-TAP1 (clone 148.3) 32, anti-TAP2 (clone 438.3) 33, anti-tapasin (clone 7F6) 34, anti-HLA-A/B/C (clone W6/32, BioLegend ... ponniyin selvan imax ticket booking chennaiWebDec 28, 2024 · Mouse BM cells were stained with CD45-AlexaFluor700 (BioLegend, clone H130), HLA-A,B,C-PE/Cy7 (BioLegend, clone W6/32), CD33-PE (BioLegend, clone WM53), and CD19-BV605 (BD Biosciences, clone SJ25C1). ponniyin selvan film downloadWebJul 8, 2024 · MHC class I Antibody (W6/32) is a mouse monoclonal IgG 2a κ MHC class I antibody, cited in 32 publications, provided at 200 µg/ml; MHC class I Antibody (W6/32) is recommended for detection of HLA-A, HLA-B and HLA-C of human origin by WB, IP, IF and FCM; recognizing the W6/32 antigenic determinant common to HLA-A, B and C antigens … shaolin eagle clawWebOct 1, 2024 · Target cell surface antigens were stained for 30 minutes prior incubation with NK cells, using the following antibodies: anti-human HLA-A, -B, -C-Brilliant Violet 605 (BioLegend; clone: W6/32), anti-human MICA/MICB-APC (BioLegend; clone: 6D4) and anti-human PD-L1-Brilliant Violet 605 (BioLegend; B7-H1, CD274) antibodies. shaolin drunk monkey 1981WebJan 21, 2024 · Clone W6/32 recognizes residues in the N terminus of the human 2-microglobulin molecule 21 . Additional reported applications (for the relevant formats) … ponniyin selvan hindi near me