Phospho p53bp1 fluorescent staining

Author: cnhm

August undefined, 2024

WebFigure 1. Phospho-specific Antibody Arrays Referenced in Primary Literature. There are over 200 references to phospho-specific, membrane-based antibody arrays in the primary … WebWestern blot analysis of extracts from Jurkat cells, untreated (-) or treated with LY294002 #9901 or Calyculin A #9902 (+), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (upper), Akt (pan) (C67E7) Rabbit mAb #4691 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). The western blot membranes were treated with our standard antibody diluent and …

Phospho-p53 (Ser15) Antibody #9284 - Cell Signaling Technology

WebStaining Surface Molecules: Prepare a cocktail of fluorescent antibodies specific for staining cell surface antigens in 1× TFP Perm/Wash Buffer or Stain Buffer (BSA). For example, dilute fluorescent surface antibodies at a 10 µL:1000 µL dilution to make a cocktail of 50 tests at 20 µL per test. 14. Incubate for 40-50 min at 2-8ºC . 15. sims school log in

Phospho-p95/NBS1 (Ser343) Antibody Cell Signaling Technology

WebImmunofluorescence staining was performed as described previously . Antibodies against 53BP1, Chk2, Chk2T68P, and γ-H2AX were generated as described previously ( 22 , 26 , 27 … WebBioss Anti-Phospho-p53BP1 (Ser25, Ser29) Polyclonal, Catalog # BS-3020R. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Frozen) (IHC (F)), … WebParaformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p53BP1 (Ser25 + Ser29)) Polyclonal Antibody, Unconjugated at … rcs saft

Phospho-p53 (Ser15) Antibody Cell Signaling Technology

Anti-phospho-53BP1 (Thr1609/Ser1618) Antibody

WebStaining Surface Molecules: Prepare a cocktail of fluorescent antibodies specific for staining cell surface antigens in 1× TFP Perm Wash Buffer or Stain Buffer (BSA). For … WebCheck the product information for recommendations for each primary antibody being used. Preheat the antigen retrieval buffer (100 mM Tris, 5% [w/v] urea, pH 9.5) to 95°C. This can … rcs schipholWebJun 22, 2001 · Whereas acetylated at G 0, p53 was found to exhibit phosphorylation on residues 15, 20, and 372 at the G 1 phase of the cell cycle and on aa 37 and 392 during the … sims school census

"WebIdentify the problem with your immunofluorescence staining from the options below: Weak or No Staining High Background Non-specific Staining Weak or No Staining Incorrect light source/filter set: Ensure your microscope is equipped with the correct light source and filter set for the fluorophore you have chosen. Gain/exposure is too low: " - Phospho p53bp1 fluorescent staining

Phospho p53bp1 fluorescent staining

(PDF) Ginsenoside Rg3 regulates DNA damage in non-small cell …

WebThe p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and … WebRepair of radiation-induced DNA double-strand breaks is dependent on the multifunctional MRN complex containing Mre11, Rad50, and the NBS1 gene product p95/NBS1 (also …

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WebInvitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. ... Invitrogen BODIPY dyes have unique hydrophobic ... WebNov 1, 2024 · ZGW attenuated podocyte apoptosis, and oxidative stress was detected by the peroxide-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) staining in a dose-dependent manner. Furthermore, ZGW decreased the expression of caspase-3 and phospho-p38 in both the kidney cortex and high glucose-treated podocytes.

WebNotably, the novel emanating functions of pKu70 are evidenced through the recruitment of RNA Pol II and concomitant formation of phospho-53BP1 foci. Phosphorylation is also a prerequisite for the dynamic release of Ku70 from the repair complex through neddylation-dependent ubiquitylation. WebMar 24, 2024 · In contract with this hypothesis will be the functions repporting an inhibition of cNHEJ in G2 by CYREN or by phospho-RECQ4 (60,61). The data of RNA Pol II recruitment by pKu70 and the forming of p53BP1 foci is obviously the central locating of this research, since -irradiation induces multiple types of DNA ends (filthy DSBs).

WebThe stain can be used with standard SDS-polyacrylamide gels or with 2-D gels. Features High sensitivity. Detect as little as 1 ng phosphoprotein. Simple and fast staining. Compatibility with standard laboratory equipment. Wide linear detection range. At least three orders of magnitude. WebJul 30, 2024 · We found that 53BP1 is already significantly phosphorylated at mild dose of HU ( Fig 1C) and accumulates in foci in replicating cells only 30 minutes after induction of DRS ( S1B and S1C Fig ). Importantly, because we extracted soluble proteins before cell fixation, we only quantified proteins on chromatin and therefore DSB bound p53BP1.

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WebPhospho-53BP1 (Ser1778) Antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 1778. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic … sims school register downloadWebApr 20, 2006 · Increasing the number of cells to 5 million per stain increased the coefficient of variation (CV) of phospho-antibody staining 3–5%, with CVs typically in the range of 50–70% (Supplementary ... rcs san rafaelWebAfter washing the cells twice with BD Pharmingen™ Stain Buffer (Cat. No. 554656/554657), the PBMC were stained with Alexa Fluor® 488 Rat Anti-ERK1/2 (pT202/pY204) (Cat. No. 512592). ... Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer II can be used with antibodies for phospho-protein analysis (i.e the ... rcss arr atisWebDec 25, 2024 · We describe a method for detecting phosphoproteins by dot-blot staining with a Phos-tag Aqua fluorescent dye. By using the method, three types of in vitro protein kinase assays for visualizing Tyr ... rcs school supply listWebPhospho-53BP1 (Thr543) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan ®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Thr543 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. rcss - blythe elementary schoolWebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). sims school register freeWebThe staining patterns of 53BP1 nuclear foci and 53BP1 mRNA level are associated with cervical cancer progression and metastasis. p53-binding protein 1 (53BP1) plays a key … sims school login teacher